Summary: These data serve as a reference transcriptomic profile for adult mouse retinal Muller glia. Retinas from 35-week-old mice were dissociated into single cell suspensions and two fractions were collected by fluorescence activated cell sorting: a Muller glia-enriched fraction based on Muller glia-specific tdTomato expression and a second fraction containing the remaining retinal cells that were tdTomato-negative. tdTomato expression from the Rosaai14 allele was activated with the Rlbp1:CreERT2 transgene following tamoxifen treatment. 3 biological replicates from the tdTomato-positive fraction and 2 from the tdTomato-negative fraction passed QC measures and were sequenced.

Protocol details: Retinas were dissected from adult mice and dissociated using the Papain Dissociation Kit (Worthington). Total RNA was isolated using TRIzol reagent (Invitrogen). mRNA libraries were prepared with poly-A selection and the Clontech SMARTer low input RNA library kit (Takara) and sequenced (paired-end 150 bp) using a NovaSeq6000 platform (Illumina). Adapter sequences were removed using Cutadapt v1.16. Reads were aligned to the GENCODE GRCm38.p5 genome using STAR v2.5.3a. GENCODE vM12 gene annotations were provided to STAR to improve the accuracy of mapping. FeatureCounts v1.15.29 was used to produce count matrices. Normalization and differential expression were done with DESeq2 v1.18.1.

Data access: ArrayExpress E-MTAB-7058