Mouse Retinal Development Single Cell RNAseq Analysis

Hyde Lab

Hyde Lab

Jun 18, 2019


RNA Sequencing
Single Cell Sequencing

Precise temporal control of gene expression in neuronal progenitors is necessary for correct regulation of neurogenesis and cell fate specification. However, the cellular heterogeneity of the developing CNS has posed a major obstacle to identifying the gene regulatory networks that control these processes. To address this, we used single-cell RNA sequencing to profile ten developmental stages encompassing the full course of retinal neurogenesis. This allowed us to comprehensively characterize changes in gene expression that occur during initiation of neurogenesis, changes in developmental competence, and specification and differentiation of each major retinal cell type. We identify the NFI transcription factors (Nfia, Nfib, and Nfix) as selectively expressed in late retinal progenitor cells and show that they control bipolar interneuron and Müller glia cell fate specification and promote proliferative quiescence. UMAP-dimension reduction of droplet-based single cell RNA-sequencing of developing mouse retina, with doublets and extra-retinal cells removed. Data published in Single cell RNA-Seq analysis of retinal development identifies NFI factors as regulating mitotic exit and late-born cell specification, described in Figure 1(E&F). Study conducted by Dr. Seth Blackshaw with the support of the NEI's Audacious Goals Initiative. Correspondence should be addressed to Dr. Seth Blackshaw

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